This study aims to develop a comprehensive framework to evaluate the service ability of agri-food supply chain based on Chinese food industry. We develop an evaluation index system with five aspects resepectively, transportation, wareshousing & storage, customer response, distribution and information level. With analytic hierarchy process to identify the weight of each index, we apply fuzzy comprehensive evaluation method to evaluate the overall system. A numberic example shows that the evaluating system can be applied not only for agricultural enterprises to select cold chain service suppliers, but also for these suppliers to analyze their own service ability to identify the oppertunities for improvement.