文章基本信息

标题：Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy
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作者：Selene Marozzi ; Paola De Santis ; Sarah Lovari 等
期刊名称：Italian Journal of Food Safety
电子版ISSN：2239-7132
出版年度：2016
卷号：5
期号：1
DOI：10.4081/ijfs.2016.4566
语种：English
出版社：PAGEPress Publications
摘要：In recent years, the incidence of foodborne diseases caused by Shiga toxin-producing Escherichia coli (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteurised milk cheeses was investigated. In total, 203 samples obtained from March 2011 to December 2013 were analysed, with two standard methods (ISO 16654:2001 and ISO 13136:2012). Two strains of E. coli O157 were isolated (2/161, 1.2%) but did not carry any virulence-associated genes and 22 stx -positive samples (22/146, 15.1%) were detected in enrichment cultures, mostly from ovine cheeses. Only two strains isolated from different ovine cheeses carried stx gene and none of these was eae -positive. This study confirms the presence of stx -positive E. coli and suggests that this type of food cannot be excluded as a potential vehicle of STEC.
关键词：Shiga-toxin-producing Escherichia coli;Raw milk cheeses;Prevalence