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标题：Prevalence of Legionella in domestic hot water systems in homes in France
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作者：France Wallet ; Corinne Emery ; Emmanuel Briand 等
期刊名称：Environnement, Risques & Santé
印刷版ISSN：1635-0421
电子版ISSN：1952-3398
出版年度：2016
卷号：15
期号：1
页码：29-38
DOI：10.1684/ers.2016.0835
出版社：John Libbey Eurotext
摘要：Tables Authors France Wallet ¹ * Corinne Emery ² Emmanuel Briand ³ Pierre-André Cabanes ¹ 1 EDF Service des études médicales 45, rue Kléber 92309 Levallois-Perret Cedex France 2 Cemka-Eval 43, boulevard du Maréchal Joffre 92340 Bourg-la-Reine France 3 Direction générale de la santé 8, avenue de Ségur 75350 Paris 07 SP France * Reprints Key words: Legionella, prevalence, amoebae, water microbiology, cell culture technique, image cytometry DOI : 10.1684/ers.2016.0835 Page(s) : 29-38 Published in: 2016 This study of 141 homes in three regions of France sought to determine the prevalence of Legionella in water production and distribution systems in homes and to identify factors associated with contamination. Descriptive data were collected about the homes and the water systems. Physicochemical and microbiological analyses included investigations for amoebae and Legionella in hot, cold, and mixed water. Legionella were counted by plate culture and solid phase cytometry with immunofluorescent labelling. The prevalence of Legionella by plate culture was 5% in hot water and 5.6% in mixed water (threshold 1000 CFU/L – CFU: Colony forming unit); it increased to 41% in hot water, 52% in mixed water, and 53% in cold water (threshold 1000 CFU/L) when assayed by immunocytometry. Factors associated with the presence of Legionella were the origin of the water (surface) (OR=38), presence of amoebae (OR=5.7), and presence of a water softener or filter (OR=11.1). Only the association with surface water was significant.
关键词：Legionella; prevalence; amoebae; water microbiology; cell culture technique; image cytometry