文章基本信息

标题：Subtyping of HIV-1 BY gag heteroduplex mobility assay
本地全文：下载
作者：Santanu Das ; Srikanth Tripathy
期刊名称：International Journal of Fundamental and Applied Sciences
印刷版ISSN：2278-1404
出版年度：2015
卷号：4
期号：2
页码：30-36
出版社：BioMedAsia
摘要：Surveillance of the different HIV-1 subtypes has important implications for developing candidate vaccines and understanding the dynamics of HIV-1 transmission in various populations. In this study, HIV-1 viral subtypes were determined for homologies in p24 region by the heteroduplex mobility assay (HMA) in 124 patients and by sequencing of 'gag gene' with sexually transmitted diseases (STD) in selected places all over India. Methodology: Proviral DNA from whole blood and from PBMC's from recent sero-converters and seropositive individuals were analyzed. Results: Of 121 samples analyzed, 115 (95%) were HIV-1 C subtype, 5(4%) were HIV-1 A subtype and 1(1%) was HIV-1 B subtype. Further analysis revealed that 114 (99%) of the C subtype samples had maximum homology to the C6-Indian reference strain, while 1 (1%) was most homologous to the C3-Zambian strain. Conclusion: These findings have important implications for the design and testing of effective candidate HIV-1 vaccines in India.
关键词：HIV-1; heteroduplex mobility assay; AIDS; STD