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标题：Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds
其他标题：Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds
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作者：POMPEU, Dávia Guimarães ; MATTIOLI, Marcelo Augusto ; RIBEIRO, Rosy Iara Maciel de Azambuja 等
期刊名称：Food Science and Technology (Campinas)
印刷版ISSN：0101-2061
电子版ISSN：1678-457X
出版年度：2015
卷号：35
期号：4
页码：696-703
DOI：10.1590/1678-457X.6823
出版社：Sociedade Brasileira de Ciência e Tecnologia de Alimentos
摘要：Abstract A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.
其他摘要：Abstract A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.
其他关键词：antimicrobial;Chenopodium quinoa;glucose/mannose-specific;lectin;seeds