文章基本信息

标题：Design of a single protein that spans the entire 2-V range of physiological redox potentials
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作者：Parisa Hosseinzadeh ; Nicholas M. Marshall ; Kelly N. Chacón 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2016
卷号：113
期号：2
页码：262-267
DOI：10.1073/pnas.1515897112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The reduction potential (E°′) is a critical parameter in determining the efficiency of most biological and chemical reactions. Biology employs three classes of metalloproteins to cover the majority of the 2-V range of physiological E°′s. An ultimate test of our understanding of E°′ is to find out the minimal number of proteins and their variants that can cover this entire range and the structural features responsible for the extreme E°′. We report herein the design of the protein azurin to cover a range from +970 mV to −954 mV vs. standard hydrogen electrode (SHE) by mutating only five residues and using two metal ions. Spectroscopic methods have revealed geometric parameters important for the high E°′. The knowledge gained and the resulting water-soluble redox agents with predictable E°′s, in the same scaffold with the same surface properties, will find wide applications in chemical, biochemical, biophysical, and biotechnological fields.
关键词：reduction potential ; secondary coordination sphere ; azurin ; electron transfer ; cupredoxins