文章基本信息

标题：Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity
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作者：Prabhat K. Singhal ; Slim Sassi ; Lan Lan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2016
卷号：113
期号：1
页码：122-127
DOI：10.1073/pnas.1522401112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiated from different embryonic organs, and cells sorted according to their surface phenotype typically retain their characteristics on continued propagation in culture. Similarly, surface phenotypes of individual cloned fibroblast-like cells exhibit significant variation. The fibroblast cell class appears to contain a very large number of denumerable subtypes.
关键词：embryo ; fibroblast ; lineage ; heterogeneity ; phenotype