文章基本信息

标题：Chemical tools for the study of hydrogen sulfide (H2S) and sulfane sulfur and their applications to biological studies
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作者：Yoko Takano ; Kazuhito Shimamoto ; Kenjiro Hanaoka 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2016
卷号：58
期号：1
页码：7-15
DOI：10.3164/jcbn.15-91
出版社：The Society for Free Radical Research Japan
摘要：Hydrogen sulfide (H₂S) functions in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. On the other hand, sulfane sulfur, which is a sulfur atom with six valence electrons but no charge, has the unique ability to bind reversibly to other sulfur atoms to form hydropersulfides (R-S-SH) and polysulfides (-S-S_n-S-). H₂S and sulfane sulfur always coexist, and recent work suggests that sulfane sulfur species may be the actual signaling molecules in at least some biological phenomena. For example, one of the mechanisms of activity regulation of proteins by H₂S is the S -sulfhydration of cysteine residues (protein Cys-SSH). In this review, we summarize recent progress on chemical tools for the study of H₂S and sulfane sulfur, covering fluorescence probes utilizing various design strategies, H₂S caged compounds, inhibitors of physiological H₂S-producing enzymes (cystathionine γ-lyase, cystathionine β-synthase and 3-mercaptopyruvate sulfurtransferase), and labeling reagents. Fluorescence probes offer particular advantages as chemical tools to study physiological functions of biomolecules, including ease of use and real-time, nondestructive visualization of biological processes in live cells and tissues.
关键词：hydrogen sulfide;sulfane sulfur;fluorescence probe;caged compound;enzyme inhibitor