文章基本信息

标题：Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli
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作者：Chonglong Wang ; Liyang Yang ; Asad Ali Shah 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep16505
出版社：Springer Nature
摘要：Engineering of efflux pumps is a promising way to improve host’s tolerance to biofuels such as medium-chain alcohols (C_mOHs); however, this strategy is restricted by poor understanding of the efflux pumps engaged in extrusion of solvents. In this study, several Escherichia coli mutants of multidrug transporters were evaluated for isoprenol tolerance. Susceptible phenotypes were observed in the mutants with individual deletion of six transporters, AcrD, EmrAB, MacAB, MdtBC, MdtJI and YdiM, whereas inactivation of AcrAB transporter resulted in an improved tolerance to isoprenol and other C_mOHs. AcrAB is the major transporter forming tripartite transperiplasmic complex with outer membrane channel TolC for direct extrusion of toxic molecules in E . coli . The AcrAB inactivation enables to enhance TolC availability for the multidrug transporters associated with extrusion of C_mOHs and increase the tolerance to C_mOHs including isoprenol. It is assumed that outer membrane channel TolC plays an important role in extrusion of isoprenol and other C_mOHs.