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标题：Evaluation of CD40 and CD80 receptors in the colonic mucosal membrane of children with inflammatory bowel disease
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作者：Barbara Kamińska ; Izabela Roszko-Kirpsza ; Piotr Landowski 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：4
页码：695-699
DOI：10.5604/12321966.1185778
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. The most prevalent inflammatory bowel diseases (IBD) include ulcerative colitis (UC) and Crohn’s disease(CD). Immune processes play a vital role in the etiopathogenesis of these conditions, involving both cellular and humoralresponse mechanisms. The aim of this study was to quantify CD40- and CD80-positive cells in the biopsy specimens oflarge intestinal mucosa from children with IBD.Materials and method. The study comprised 38 children aged between 3–17 years (mean 11.5±3.7 years) – 20 boys (52.6%)and 18 girls (47.4%). Eighteen patients were diagnosed with UC on the basis of clinical manifestation, endoscopic andhistopathological findings. Mean age of this subgroup was 11.55±4.07 years. A group of 10 children (mean age 12.30±2.83)diagnosed with CD was also included. The control group comprised 10 IBD-free children (mean age 10.28±4.07 years). Thesurface expressions of CD40 and CD80 were analyzed in large intestine mucosa biopsy specimens, fixed in formaldehyde,embedded in paraffin, and cut with a microtome into 4 μm slices.Results. The number of CD40- and CD80-positive cells in the large intestinal mucosa of children with Crohn’s disease andulcerative colitis was significantly higher than in the controls. The highest number of CD40+ and CD80+ cells was observedin the caecal mucosal membrane of Crohn’s disease patients and in the rectal mucosa of individuals with ulcerative colitis.Conclusion. IBD is characterized by elevated, segment-specific, expression of CD40 and CD80
关键词：inflammatory bowel disease; children; CD40; CD 80 receptors