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标题：Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis
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作者：Krzysztof Stojecki ; Jacek Sroka ; Tomasz Cencek 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：4
页码：594-598
DOI：10.5604/12321966.1185759
出版社：Institute of Agricultural Medicine in Lublin
摘要：AbstractFaecal samples from 297 farm animals were collected from 18 households in distinct sites of the Łęczyńsko-WłodawskieLake District of eastern Poland. They included samples from 86 cattle (Bos taurus), 84 pigs (Sus scrofa f. domestica), 81 sheep(Ovis aries), 10 horses (Equus caballus), and 36 dogs (Canis lupus familiaris). The samples were examined for the presence ofGiardia intestinalis by the Direct Fluorescence Assay (DFA) and semi-nested PCR. All amplicons were sequenced on bothstrands. By DFA, cysts of Giardia spp. were detected in 66 of 297 faecal samples (22.2%). Positive specimens for Giardia spp.were derived from 29.8% of examined pigs, 21.0% of sheep, 18.6% of cattle, 10% of horses, and 19.4% of dogs. Based on thedetection of the β-giardin gene by PCR, 39 (13.1%) of the 297 examined samples were recognized as positive. Detection ofthe presence of Giardia cysts by DFA test was overall significantly higher compared to PCR (p=0.0045). By PCR, Giardia wasfound in 28.1% of sheep, 11.6% of cattle, 10% of horses, 9.5% of pigs and 5.6% of dogs. Partial β-giardin gene sequenceswere obtained for 73.7% of the PCR positive samples. From sequenced samples derived from the studied animals, Giardiawere identified as assemblage A (8 samples), B (1 sample) and E (18 samples). As assemblages A and B may be zoonotic, thefarm animals living in eastern Poland could be regarded as a potential source of Giardia infection for humans
关键词：Giardia intestinalis; farm animals; faecal samples; eastern Poland; Direct Fluorescence Assay; PCR