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标题：Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater
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作者：Tatiana Čornejová ; Jan Venglovsky ; Gabriela Gregova 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2015
卷号：22
期号：3
页码：447-450
DOI：10.5604/12321966.1167710
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction and objective. Over the past decades, awareness of the environmental load of resistant organisms hasincreased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coliand on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated froman urban wastewater treatment plant.Materials and method. Wastewater samples and artificially added polystyrene pellets were used as the source for E. coliisolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formationwas investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, qnrS, mobile elements and virulencefactors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldibiotyper.Results. ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and qnrS genes of antibioticresistance were detected. IMP gene together with integron 1 in one ertapenem resistant E. coli was also recorded. Therewas no evident correlation between antibiotic resistance, virulence and biofilm production.Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinoloneresistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, qnrS genes and virulence factorspresent a potential environmental health risk.
关键词：E.coli; ESBL; virulence; biofilm