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  • 标题:Review of methods used for identification of biothreat agents in environmental protection and human health aspects
  • 本地全文:下载
  • 作者:Tomasz Mirski ; Michał Bartoszcze ; Agata Bielawska-Drózd
  • 期刊名称:Annals of Agricultural and Environmental Medicine
  • 印刷版ISSN:1232-1966
  • 电子版ISSN:1898-2263
  • 出版年度:2014
  • 卷号:21
  • 期号:2
  • 页码:224-234
  • DOI:10.5604/1232-1966.1108581
  • 出版社:Institute of Agricultural Medicine in Lublin
  • 摘要:Modern threats of bioterrorism force the need to develop methods for rapid and accurate identification of dangerousbiological agents. Currently, there are many types of methods used in this field of studies that are based on immunologicalor genetic techniques, or constitute a combination of both methods (immuno-genetic). There are also methods that havebeen developed on the basis of physical and chemical properties of the analytes. Each group of these analytical assays canbe further divided into conventional methods (e.g. simple antigen-antibody reactions, classical PCR, real-time PCR), andmodern technologies (e.g. microarray technology, aptamers, phosphors, etc.). Nanodiagnostics constitute another groupof methods that utilize the objects at a nanoscale (below 100 nm). There are also integrated and automated diagnosticsystems, which combine different methods and allow simultaneous sampling, extraction of genetic material and detectionand identification of the analyte using genetic, as well as immunological techniques
  • 关键词:diagnostic techniques; bioterrorism; immunological methods; genetic methods
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