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标题：Assessment of viability of the nematode eggs ( Ascaris , Toxocara , Trichuris ) in sewage sludge with the use of LIVE/DEAD Bacterial Viability Kit
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作者：Joanna Dąbrowska ; Jolanta Zdybel ; Jacek Karamon 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：1
页码：35-41
出版社：Institute of Agricultural Medicine in Lublin
摘要：Sewage sludges from wastewater treatment plants may contain live parasite eggs, which can be a source of humansand animals infection. According to the current rules, parasitological examination includes detection of the Ascaris spp.,Trichuris spp. and Toxocara spp. eggs and estimation of their viability. The viability assessment based only on the incubationand observation of isolated egg is long and imprecise. The aim of this study was to develop sensitive and less labourintensivemethods for assessing viability of Ascaris spp., Toxocara spp. and Trichuris spp. eggs in sewage sludge. For thispurpose, LIVE/DEAD Kit was used. Firstly, the possibility of distinguishing between live and dead eggs in water was assessed.Secondly, an appropriate amount of dyeing mixture needed to distinguish the live and dead eggs in the sewage sludge wasdetermined using experimentally enriched samples and naturally contaminated samples of sludge. Eggs were isolated fromthe samples by own method which was a combination of flotation and sedimentation, preceded by a long mixing. Afterthe last stage of the procedure, sediment containing the eggs of parasites was stained by LIVE/DEAD kit according to themanufacturer instructions, but with the use of different variants of dyes mixture concentration. The investigation showedthat live and dead eggs of these three parasites could be differed by this method with the use of proper concentration ofdyes. Live eggs were stained in green (Ascaris and Trichuris) and green-blue (Toxocara). However, all types of dead eggswere red coloured. The study demonstrated that after some modifications (resulted from the nature of the samples) theLIVE/DEAD kit is useful for assessing the viability of Toxocara, Ascaris and Trichuris eggs occurring in the sludge.
关键词：Parasite eggs; sewage sludge; wastewater treatment; fluorescence