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标题：Detection of the polyphenolic components in Ribes nigrum L.
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作者：Monica BUTNARIU
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2014
卷号：21
期号：1
页码：11-14
出版社：Institute of Agricultural Medicine in Lublin
摘要：Background. The blackcurrant (Ribes nigrum L.) is a species of native currant which contains a lot of polyphenolic antioxidantswhich is used medicinally and has a fundamental role in the maintenance health.Materials, methods and objective. Ultraviolet–visible spectrophotometry and ultraviolet range high performance liquidchromatography (HPLC) were used to characterize the polyphenolic content of common Ribes nigrum collected in thewestern part of the Banat Region in Romania.Results. UV–visible spectrophotometry was a reliable tool for identifying the phenolic compounds class. Polyphenolscalibration curves from the methanolic extracts showed a good linearity (r2>0.984) within test ranges and generated a well–designed absorption band with a local maximum at 273.2 nm band, which can be attributed to thr electronic transition of then–p* type. Chromatographic separation and analysis of the methanol extract was useful for the structural epigallocatechin(EGC) and epigallocatechin–3–gallate (EGCG) characterization of primary antioxidant compounds.Conclusions. The new, slightly modified, chromatographic system can serve for the development of a quantitative assessmentmethodology of epigallocatechin and epigallocatechin–3–gallate compounds, as well as for the comparative characterisationand standardisation of the dominant polyphenolic components in Ribes nigrum using EGC and EGCG standards
关键词：UV–visible spectrophotometry; HPLC; polyphenolic components