This article analyses the relationship between the number of hours spent by university students on the Internet and their attitude, training, use, impact and perception of difficulties in Web 2.0 integration, as well as their knowledge and use of Web 2.0 tools in Higher Education. To this end, we used a Likert scale applied to a sample of 403 students from the University of Huelva (UHU), Spain. To test the hypothesis, we conducted a one-way ANOVA with post hoc comparisons. The results obtained partially confirmed the general hypothesis. The greatest differences were found in the factors of impact and use of Web 2.0 tools, whereas the least differences were found in the factors of attitude, training, difficulties and knowledge of Web 2.0 tools.