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标题：Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route
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作者：H. Hermansyah ; M. B. Faiz ; R. Arbianti 等
期刊名称：International Journal of Environmental Science and Development
印刷版ISSN：2010-0264
出版年度：2015
卷号：6
期号：6
页码：439-444
DOI：10.7763/IJESD.2015.V6.633
摘要：AbstractUtilizing whole-cell biocatalyst is a potential way to reduce catalyst cost in biodiesel production using lipase as catalyst. Whole-cell of Rhizopus oryzae was cultivated by one-step and two-step method and was immobilized on Biomass Support Particles (BSPs) and chitosan-TPP bead. Immobilized whole-cells on BSPs produce 11% (one-step) and 12% (two-step) FAME yield. While, FAME yield produced by immobilized whole-cell in chitosan-TPP beads are 23% (one-step) and 22% (two-step). Kinetic model based Michaelis-Menten used was found to fit fairly the substrate and product concentration profile. Value of Km and Vmax for R. oryzae whole-cell immobilized on BSP are 4 mole L-1, 0.05 mole L-1 h-1 (one-step) and 3 mole L-1, 0.04 mol L-1 h-1 (two-step). While, for immobilized whole-cell in chitosan-TPP bead, the values are 0.3 mole L-1, 0.01 mole L-1 h-1 and 0.2 mole L-1, 0.008 mole L-1 h-1 for single-step and two-step respectively.
关键词：Biodiesel; immobilization; Michaelis; Menten; whole; cell