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标题：Aged iPSCs display an uncommon mitochondrial appearance and fail to undergo in vitro neurogenesis
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作者：Andrea Masotti ; Antonella Celluzzi ; Stefania Petrini 等
期刊名称：Aging
出版年度：2014
卷号：6
期号：12
页码：1094-1108
出版社：U.S.Department of Health & Human Service
摘要：Reprogramming of human fibroblasts into induced pluripotent stem cells (iPSCs) leads to mitochondrial rejuvenation, making iPSCs a candidate model to study the mitochondrial biology during stemness and differentiation. At present, it is generally accepted that iPSCs can be maintained and propagated indefinitely in culture, but no specific studies have addressed this issue. In our study, we investigated features related to the 'biological age' of iPSCs, culturing and analyzing iPSCs kept for prolonged periodsin vitro. We have demonstrated that aged iPSCs present an increased number of mitochondria per cell with an altered mitochondrial membrane potential and fail to properly undergo in vitro neurogenesis. In aged iPSCs we have also found an altered expression of genes relevant to mitochondria biogenesis. Overall, our results shed light on the mitochondrial biology of young and aged iPSCs and explore how an altered mitochondrial status may influence neuronal differentiation. Our work suggests to deepen the understanding of the iPSCs biology before considering their use in clinical applications.
关键词：induced pluripotent stem cells; mitochondria; stem cell aging; mitochondrial dysfunction; in vitro neurogenesis