Nowadays, there has been an increasing interest in the preservation of natural dentition and the proper occlusion related to tooth wear for quality of life. To overcome the problems of the existing qualitative tooth wear analysis method, virtual three-dimensional models have been used. This study was designed to develop and validate a new quantitative method using tooth wear measurement parameters with angles obtained from virtual vectors and planes of the three-dimensional models. Sixteen parameters were evaluated in the virtual models of 20 students (7.57±1.55 years old) and 20 adults (56.85±6.34 years old). There were 12 angle and 4 height parameters, and the number of parameters measured from the virtual planes and vectors were 10 and 6, respectively. For each parameter, means and standard deviations were calculated, and an unpaired sample t test was performed to compare the young and the adult groups. Also, differences between the means were determined and expressed as percentages. The results were statistically significant between the two groups ( P <0.001). In general, parameters using virtual vectors showed greater change than virtual plane. Although there were statistically significant differences among all parameters using virtual planes ( P <0.001), the changes of the three angles were similar, except distolingual cusp angle. It was found that the parameters using virtual vectors were effective and tooth wear took place in both buccal and lingual cusps. Likewise, the validation of the new measurement parameters suggests that they can also be applied in the assessment of tooth wear related to dental biomaterials.