文章基本信息

标题：Proteomics Tools for the Contemporary Identification of Proteinaceous Binders in Gilded Samples
本地全文：下载
作者：S. Hrdlickova Kuckova ; J. Schultz ; R. Veiga 等
期刊名称：International Journal of Conservation Science
印刷版ISSN：2067-533X
电子版ISSN：2067-8223
出版年度：2015
卷号：6
期号：Special
页码：507-518
出版社：Alexandru Ioan Cuza University of Iasi
摘要：This paper deals with the characterization of several gilded and polychrome samples from the carved wood Portuguese heritage using a novel proteomics approach aimed to identify and map the presence of different proteinaceous binders. Eight samples originating from two altarpieces (Main altarpiece from Miranda de Douro Cathedral and Main altarpiece of the Cathedral in Funchal, Madeira) and three polychrome sculptures (St. Joseph from Aveiro Museum and two sculptures by Machado de Castro) were taken and analysed both as fragments/separated layers (e.g. ground layer separated from the gilded/polychrome layers) and cross-sections. The methodology proposed here makes use of proteomics techniques such as ELISA, MALDI-TOF-MS, nano-LC-ESI-Q-TOF and optical microscopy coupled with a fluorescent staining test (Sypro Ruby) on samples and cross-sections and is part of the analytical task of the Gilt-Teller project (www.gilt-teller.pt). ELISA, and both mass spectrometric techniques MALDI-TOF and nano-LC-ESI-Q-TOF showed a good detection of collagens on whole fragments or separated layers and also on cross-sections, while the mass spectrometric techniques were less sensitive for egg proteins. Nevertheless, the combined use of them together with the mapping on cross-sections using the fluorescent stain made the simultaneous identification and location of these binder materials in the stratigraphy of some samples possible.
关键词：Proteomics; ELISA; LC-MS/MS; MALDI-TOF MS; proteinaceous binders; gilded carved wood