摘要：Figures Authors Stéphane Grison 1 Gaëlle Favé 2 Matthieu Maillot 2 Line Manens 1 Jean-Charles Martin 2 Marc Benderitter 1 Jocelyne Aigueperse 1 Maâmar Souidi 1 * 1 Institut de radioprotection et de sûreté nucléaire Direction de la radioprotection de l’Homme IRSN BP no 17 92262 Fontenay-aux-Roses cedex France 2 UMR INRA 1260 INSERM 1062 AIX-Marseille Université NORT Plateau BIOMET Faculté de médecine de La Timone 27, boulevard Jean Moulin 13385 Marseille France * Tirés à part Key words: cesium 137, chronic contamination, metabolom, uranium DOI : 10.1684/ers.2015.0820 Page(s) : 502-10 Published in: 2015 The IRSN ENVIRHOM-Health research program, using an experimental animal model mimicking chronic contamination of residents of contaminated territories (such as those around Chernobyl and Fukushima), showed that chronic ingestion of low-dose radionuclides results in extensive but low-amplitude biological damage, with the subtle ruptures of metabolic equilibrium that are characteristic of internal exposure. In vivo internal contamination of radionuclides can affect numerous important metabolic systems of the organism, including those of cholesterol, vitamin D, iron, steroid hormones, and xenobiotics, without producing any toxic effects or visible pathology. To complement and refine these findings, in collaboration with the broad-spectrum metabolic analysis platform of the University of Aix-Marseille (CRIBIOM), we conducted studies that combined a metabolomic approach to low-dose radiotoxicology for the first time. The results of these experimental studies, which focused on the biological effects on rats of chronic ingestion of cesium 137 and natural uranium over several months, showed modifications of the metabolome after this internal exposure. Individuals exposed to these low doses could be identified metabolomically, even though their conventional clinical markers were unaffected. Thus, a metabolomic approach using simple blood or urine analysis appears useful for defining the biological signature of internal contamination by a low radionuclide dose. Finally, the identification and the simultaneous quantification of many affected molecules defined a specific fingerprint of exposure and could help to identify exposure biomarkers, early biological effects, and toxicity leading to the eventual development of pathology. In conclusion, metabolomics is one of the modern analytical approaches with considerable potential in the area of low-dose toxicology.
Loading...
