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标题：Effects of ultraviolet C irradiation on stilbene biosynthesis in Vitis vinifera L. cv. Cabernet Sauvignon berry skins and calli
其他标题：Effects of ultraviolet C irradiation on stilbene biosynthesis in Vitis vinifera L. cv. Cabernet Sauvignon berry skins and calli
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作者：Xu J.-C. Zhan, A. ; Huang, W.-D.
期刊名称：South African Journal of Enology and Viticulture
印刷版ISSN：2224-7904
出版年度：2015
卷号：36
期号：2
页码：256-266
出版社：South African Journal of Enology and Viticulture (SAJEV)
摘要：Vitis vinifera L. cv. Cabernet Sauvignon berries and calli were irradiated with ultraviolet C (UV-C) to investigate the effects on the biosynthesis of stilbene. The stilbene content in the berry skins was enhanced significantly after 10 min of UV-C irradiation over the 24 h time course, and the results varied depending on the development stage of the fruit. The maximum production of total stilbene in the berry skins occurred at 12 h after treatment, with a content of 848.45 ± 23.53 pg/g fresh weight (FW) at the beginning of véraison, 591.77 ± 26.90 pg/g FW at the end of véraison, and 170.71 ± 6.85 pg/g FW at the ripening stage. Different UV-C dosages, from 5 to 30 min, induced stilbene accumulation considerably in the calli over the 120 h experimental period, and 20 min was the most efficient. The maximum of total stilbene accumulation in the calli was 125.07 ± 3.01 pg/g FW at 96 h after 20 min irradiation. Total phenolics and total flavonoid content increased after UV-C irradiation in both the berry skins and calli. The relative expression of genes encoding enzymes involved in the branching point of stilbene and flavonoid biosynthesis was up-regulated by UV-C irradiation. The results show that UV-C irradiation significantly promotes stilbene and flavonoid biosynthesis in grape berry skins and calli, and the induction effects depend on fruit development stage and UV-C dosage.
其他摘要：Vitis vinifera L. cv. Cabernet Sauvignon berries and calli were irradiated with ultraviolet C (UV-C) to investigate the effects on the biosynthesis of stilbene. The stilbene content in the berry skins was enhanced significantly after 10 min of UV-C irradiation over the 24 h time course, and the results varied depending on the development stage of the fruit. The maximum production of total stilbene in the berry skins occurred at 12 h after treatment, with a content of 848.45 ± 23.53 pg/g fresh weight (FW) at the beginning of véraison, 591.77 ± 26.90 pg/g FW at the end of véraison, and 170.71 ± 6.85 pg/g FW at the ripening stage. Different UV-C dosages, from 5 to 30 min, induced stilbene accumulation considerably in the calli over the 120 h experimental period, and 20 min was the most efficient. The maximum of total stilbene accumulation in the calli was 125.07 ± 3.01 pg/g FW at 96 h after 20 min irradiation. Total phenolics and total flavonoid content increased after UV-C irradiation in both the berry skins and calli. The relative expression of genes encoding enzymes involved in the branching point of stilbene and flavonoid biosynthesis was up-regulated by UV-C irradiation. The results show that UV-C irradiation significantly promotes stilbene and flavonoid biosynthesis in grape berry skins and calli, and the induction effects depend on fruit development stage and UV-C dosage.
其他关键词：Grape;calli;ultraviolet C;stilbene;flavonoid