文章基本信息

标题：Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates
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作者：Peter Owens ; Nigel Phillipson
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2015
卷号：5
期号：4
页码：664-677
DOI：10.3390/bios5040664
语种：English
出版社：MDPI Publishing
摘要：In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.
关键词：Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker Raman spectroscopy ; protein sensing ; surface-enhanced Raman scattering ; biomarker