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标题：Functional variation of potassium, sodium and chloride ions in selected salttolerant-calli from durum wheat (Triticum durum Desf.) mature embryo
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作者：Koutoua A. ; Hmouni D. ; Elyacoubi H. 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2015
卷号：6
期号：5
页码：1285-1291
出版社：University of Mohammed Premier Oujda
摘要：Mature embryos were used to establish callus cultures in MS modified medium. Cell lines calli were then submitted to increasing concentrations [0, 4, 8, 12, 16 g.L-1] of NaCl. Therefore growth, water and ions contents (K + , Na + and Cl - ) were determined in both the control (unselected wild-type calli maintained in salt-free medium) and selected (tolerant and sensitive genotypes) calli. Selected salt-tolerant calli showed no growth red uction in comparison with the control when treated with 16 g.L-1 NaCl while a significant gro wth decrease was noted in salt-sensitive ones. Water content was significantly higher in salt- tolerant calli than in salt-sensitive ones. Both tolerant and sensitive calli accumulated less K+ than the control but K+ content of salt-tolerant calli remained higher than that of salt-sensitive ones. Accumulation of Na+ and Cl- was higher in salt-sensitive calli than in salt-tolerant ones with Na+ increase being the highest. The results indicated that Na+ especially but also Cl- exclusion play a key role in salt-tolerance in wheat calli lines. The comparison of K+/Na+ ratio allowed to classify wheat varieties Sebou, Anouar and Tarek as salt-tolerant and Marzak, Ourgh, Massa, Tomouh and Amjad as salt-sensitive ones.
关键词：Triticum durum; salinity; callus; selection; salt tolerance; physiological traits