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标题：Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury
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作者：Tomoko Nakano ; Tomomi Kotani ; Yukio Mano 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2015
卷号：57
期号：3
页码：178-182
DOI：10.3164/jcbn.15-90
出版社：The Society for Free Radical Research Japan
摘要：Fetal brain injury is often related to prenatal inflammation; however, there is a lack of effective therapy. Recently, molecular hydrogen (H₂), a specific antioxidant to hydroxyl radical and peroxynitrite, has been reported to have anti-inflammatory properties. The aim of this study was to investigate whether maternal H₂ administration could protect the fetal brain against inflammation. Pregnant C3H/HeN mice received an intraperitoneal injection of lipopolysaccharide (LPS) on gestational day 15.5 and were provided with H₂ water for 24 h prior to LPS injection. Pup brain samples were collected on gestational day 16.5, and the levels of apoptosis and oxidative damage were evaluated using immunohistochemistry. Interleukin-6 (IL-6) levels were examined using real-time PCR. The levels of apoptosis and oxidative damage, as well as the levels of IL-6 mRNA, increased significantly when the mother was injected with LPS than that in the control group. However, these levels were significantly reduced when H₂ was administered prior to the LPS-injection. Our results suggest that LPS-induced apoptosis, oxidative damage and inflammation in the fetal brain were ameliorated by maternal H₂ administration. Antenatal H₂ administration might protect the premature brain against maternal inflammation.
关键词：molecular hydrogen;premature infant;anti-oxidant;anti-inflammation;brain injury