文章基本信息

标题：The Development of an Electrochemical Immunosensory for 1-naphthylacetic Acid
本地全文：下载
作者：S.Z. Liang ; J.R. Pan ; X.M. Yin 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2015
卷号：9
期号：6
页码：439-443
出版社：MAXWELL Science Publication
摘要：A new detection method for 1-naphthylacetic acid (NAA) based on the electrochemical immunosensor was introduced in the study, in which anti-NAA polyclonal antibody was used. An monolayer-molecular, self-reassemble membrane was made from 3-mercaptopropionic acid on the surface of gold electrodes, on the membrane the anti-NAA antibody was immoblize through ester-activated method with addition of 1-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Based on the three-electrode system, an NAA immunosenory detection method was established with using the signal from cyclic voltammetry. The standard curve could be represented as △E = 0.0407lgc+0.0701(R²= 0.9984) with the range from 0.05 μg/mL to 2 &mug/mL and with the detection limit was 0.035 &mug/mL. The sensory method had very low cross-reactivity, lower than 3%, to NAA similarities such as phenylacetic acid and indolyl-3-acetic acid. In addition, the sensor could be used with high stability as long as 30 days (room temperature). It indicated that a specific, stable electrochemical immunosensory detection method for 1-Naphthaleneacetic acid was successfully developed.
关键词：1-naphthylacetic acid ; electrochemical immunosensor ; self-assembled monolayer membrance ; ; ;