文章基本信息

标题：A Casparian strip domain-like gene, CASPL, negatively alters growth and cold tolerance
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作者：Jinghua Yang ; Changqing Ding ; Baochen Xu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep14299
出版社：Springer Nature
摘要：A cold-induced transcript encoding a Casparian strip membrane domain (CASP)-like protein ( ClCASPL ) was identified in watermelon ( Citrullus lanatus) . Fluorescence microscopy analysis showed that ClCASPL-GFP is localized in the plasma membrane. The orthologous gene in Arabidopsis thaliana ( AtCASPL4C1 ) was also found to play an important role in cold tolerance. Expression analysis using a β -glucuronidase (GUS) reporter reveals that AtCASPL4C1 is widely expressed in a variety of organs and is cold inducible. Analysis of AtCASPL4C1 T-DNA knock-out plants showed altered growth dynamics, faster growth, increased biomass (dry weight) and earlier flowering compared to wild type (Col-0) and ClCASPL overexpressing plants. AtCASPL4C1 knock-out plants showed elevated tolerance to cold stress, while overexpressing CICASPL resulted in increased sensitivity to cold stress in Arabidopsis . Interestingly, AtCASPL4C1 knock-out plants did not display significant alterations in the Casparian strip formation in roots. Thus, the combination of these results suggests a role for CICASPL and AtCASPL4C1 beyond Casparian strip formation in roots, possibly indicating a more fundamental role in vascular tissue.