文章基本信息

标题：Binding interface between the Salmonella σ^S/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl
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作者：Paola Cavaliere ; Christina Sizun ; Fabienne Levi-Acobas 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep13564
出版社：Springer Nature
摘要：In many Gram-negative bacteria, including Salmonella enterica serovar Typhimurium ( S . Typhimurium), the sigma factor RpoS/σ^S accumulates during stationary phase of growth, and associates with the core RNA polymerase enzyme (E) to promote transcription initiation of genes involved in general stress resistance and starvation survival. Whereas σ factors are usually inactivated upon interaction with anti-σ proteins, σ^S binding to the Crl protein increases σ^S activity by favouring its association to E. Taking advantage of evolution of the σ^S sequence in bacterial species that do not contain a crl gene, like Pseudomonas aeruginosa , we identified and assigned a critical arginine residue in σ^S to the S . Typhimurium σ^S-Crl binding interface. We solved the solution structure of S. Typhimurium Crl by NMR and used it for NMR binding assays with σ^S and to generate in silico models of the σ^S-Crl complex constrained by mutational analysis. The σ^S-Crl models suggest that the identified arginine in σ^S interacts with an aspartate of Crl that is required for σ^S binding and is located inside a cavity enclosed by flexible loops, which also contribute to the interface. This study provides the basis for further structural investigation of the σ^S-Crl complex.