文章基本信息

标题：Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate
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作者：Héctor Díaz-Maldonado ; Manuel J. Gómez ; Mercedes Moreno-Paz 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep13329
出版社：Springer Nature
摘要：Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS 1634 family in Acidiphilium over ~600 generations of cultured growth. We demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium , Leptospirillum and E. coli . Further, we also demonstrate that the Acidiphilium IS 1634 transposase binds to the archaeal sliding clamp (PCNA) from Methanosarcina , and that the transposase encoded by Methanosarcina IS 1634 binds to Acidiphilium β. Finally, we demonstrate that increasing the strength of the interaction between β and transposase results in a higher transposition rate in vivo . Our results suggest that the interaction could determine the potential of ISs to be mobilized in bacterial populations and also their ability to proliferate within chromosomes.