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标题：Modulation of protein behavior through light responses of TiO₂ nanodots films
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作者：Kui Cheng ; Yi Hong ; Mengfei Yu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep13354
出版社：Springer Nature
摘要：In this work, the behavior of protein molecules adsorbed on TiO₂ nanodots films are modulated through the light responses of the nanodots. TiO₂ nanodots films are first prepared through phase separation induced self assembly. Then, bovine serum albumin (BSA) is adsorbed on TiO₂ nanodots films and exposed to ultraviolet (365 nm) illumination. It is found the conformation of surface-bound BSA molecules changes with ultraviolet illumination. Moreover, the BSA molecules conjugate to the surface-bound molecules, which are in the overlayer, are released. The reason is ascribed to that TiO₂ nanodots absorb ultraviolet and result in the increase of surface hydroxyl groups on nanodots. Such increase further leads to intensified attraction of -NH3 groups in the surface-bound BSA molecules. That not only changes the conformation of the surface-bound BSA molecules, but also weaken the conjugation between surface-bound molecules and other BSA molecules in the overlayer. Eventually, the overlayer of BSA molecules is released. It is believed that such protein conformation variation and release behavior induced through light responses of TiO₂ nanodots are crucial in understanding the biomedical performance of TiO₂ nanostructures. Also, it could be widely utilized in tailoring of the materials-protein interactions.