文章基本信息

标题：Age-related profiling of DNA methylation in CD8+ T cells reveals changes in immune response and transcriptional regulator genes
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作者：Liina Tserel ; Raivo Kolde ; Maia Limbach 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep13107
出版社：Springer Nature
摘要：Human ageing affects the immune system resulting in an overall decline in immunocompetence. Although all immune cells are affected during aging, the functional capacity of T cells is most influenced and is linked to decreased responsiveness to infections and impaired differentiation. We studied age-related changes in DNA methylation and gene expression in CD4+ and CD8+ T cells from younger and older individuals. We observed marked difference between T cell subsets, with increased number of methylation changes and higher methylome variation in CD8+ T cells with age. The majority of age-related hypermethylated sites were located at CpG islands of silent genes and enriched for repressive histone marks. Specifically, in CD8+ T cell subset we identified strong inverse correlation between methylation and expression levels in genes associated with T cell mediated immune response ( LGALS1, IFNG, CCL5, GZMH, CCR7, CD27 and CD248 ) and differentiation ( SATB1, TCF7, BCL11B and RUNX3 ). Our results thus suggest the link between age-related epigenetic changes and impaired T cell function.