文章基本信息

标题：Molecular Signatures of Reduced Nerve Toxicity by CeCl₃ in Phoxim-exposed Silkworm Brains
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作者：Binbin Wang ; Fanchi Li ; Min Ni 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep12761
出版社：Springer Nature
摘要：CeCl₃ can reduce the damage caused by OP pesticides, in this study we used the brain of silkworms to investigate the mechanism of CeCl₃ effects on pesticide resistance. The results showed that phoxim treatments led to brain damages, swelling and death of neurons, chromatin condensation, and mitochondrial damage. Normal nerve conduction was severely affected by phoxim treatments, as revealed by: increases in the contents of neurotransmitters Glu, NO, and ACh by 63.65%, 61.14%, and 98.54%, respectively; decreases in the contents of 5-HT and DA by 53.19% and 43.71%, respectively; reductions in the activities of Na⁺/K⁺-ATPase, Ca²⁺/Mg²⁺-ATPase, and AChE by 85.27%, 85.63%, and 85.63%, respectively; and increase in the activity of TNOS by 22.33%. CeCl₃ pretreatment can significantly reduce such damages. Results of DGE and qRT-PCR indicated that CeCl₃ treatments significantly upregulated the expression levels of CYP4G 23, cyt-b 5, GSTs-σ 1, ace 1, esterase-FE 4, and β-esterase 2. Overall, phoxim treatments cause nerve tissue lesions, neuron death, and nerve conduction hindrance, but CeCl₃ pretreatments can promote the expression of phoxim resistance-related genes in silkworm brains to reduce phoxim-induced damages. Our study provides a potential new method to improve the resistance of silkworms against OP pesticides.