文章基本信息

标题：Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
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作者：Steven D. Scoville ; Karen A. Keller ; Stephanie Cheng 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep12490
出版社：Springer Nature
摘要：We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.