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标题：MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1
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作者：Hiroko P. Indo ; Tsukasa Tomiyoshi ; Shigeaki Suenaga 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2015
卷号：57
期号：2
页码：98-104
DOI：10.3164/jcbn.15-20
出版社：The Society for Free Radical Research Japan
摘要：A human neuroblastoma cell line, NB-1, was treated with 24 h of microgravity simulation by clinostat, or irradiated with extremely small X-ray doses of 0.1 or 1.0 mGy using single and 10 times fractionation regimes with 1 and 2 h time-intervals. A quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) examination was performed for apoptosis related factors ( BAX , CYTC , APAF1 , VDAC1–3 , CASP3 , CASP8 , CASP9 P53 , AIF , ANT1 and 2 , BCL2 , MnSOD , autophagy related BECN and necrosis related CYP-40 . The qRT-PCR results revealed that microgravity did not result in significant changes except for a upregulation of proapoptotic VDAC2 , and downregulations of proapoptotic CASP9 and antiapoptotic MnSOD . After 0.1 mGy fractionation irradiation, there was increased expression of proapoptotic APAF1 and downregulation of proapoptotic CYTC , VDAC2 , VDAC3 , CASP8 , AIF , ANT1 , and ANT2 , as well as an increase in expression of antiapoptotic BCL2 . There was also a decrease in MnSOD expression with 0.1 mGy fractionation irradiation. These results suggest that microgravity and low-dose radiation may decrease apoptosis but may potentially increase oxidative stress.
关键词：space radiation;microgravity;extremely small dose;neuron;NB-1