文章基本信息

标题：Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey
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作者：Zainab H. Abbas ; Kifah S. Doosh ; Nahi Y. Yaseen 等
期刊名称：Pakistan Journal of Nutrition
印刷版ISSN：1680-5194
电子版ISSN：1994-7984
出版年度：2015
卷号：14
期号：8
页码：517-523
DOI：10.3923/pjn.2015.517.523
出版社：Asian Network for Scientific Information
摘要：Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent. In this study, lactoferrin was purified from goat colostrum by ion exchange chromatography through CM-Sephadex C-50 column and gel filtration chromatography through Sephadex G-200 column. The purification fold and yield were (20.83 time and 62.50%) respectively. The purity of lactoferrin to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions ( SDS-PAGE ) with single bond. Some biochemical characteristics of the purified Lactoferrin were determined, The molecular weight of LF were 80 and 79.50kDa as determined by gel filtration and SDS-PAGE respectively . The percentage of carbohydrate content in goat Lactoferrin was 10.4%, mean while the Iron percentage was 123 ppm and as a saturated percent 7.8%.