The composting process is a useful method of producing a stabilized material that can be used as a source of nutrients and soil conditioner. Maturity of compost is essential for its optimal use as a soil amendment and a source of plant nutrients as well. Immature composts pose problems of malodors and flies and phytotoxicity and pollution during use. Stability and maturity both are required for compost quality control. Compost maturity tests can be classified into physical, chemical, plant, and microbial activity assays. In this study, several methods of evaluating the stability and maturity of composted biosolids were compared based on chemical and biological properties. The sludge used of windrow composting was obtained from the drying beds of South Isfahan wastewater treatment plant. The results showed that, C/N ratio after 100 days of composting reached to 15/1; NH₄/NO₃ ratio decreased with increase of the time dewatered sludge compost, which this loss is 57.3%. The content of volatile solids, 28.8% decreased with composting time. The number of fecal coliforms in the initial sewage sludge compost was 17.9 ¢ 10⁶ and at the end of composting was 898MPN/g of total solids and the compost process provided class A pathogen criteria. Use of chemical and biological parameters exhibited three phases: rapid decomposition (day 40), stabilization (day 80) and maturation (day 100) in biosolids compost. Thus, the biosolid compost was mature and ready for use as an agricultural substrate after about 100 days of composting.