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标题：EVALUATION OF A NATIVE PREPARATION OF HCV CORE PROTEIN (2-122) FOR POTENTIAL APPLICATIONS IN IMMUNIZATION, DIAGNOSIS AND MAB PRODUCTION
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作者：AGHASADEGHI M.R. ; SADAT S.M. ; AMINI SAFIEH 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2006
卷号：35
期号：1
页码：1-10
语种：English
出版社：THE SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES
摘要：Infection with hepatitis C virus (HCV) is a worldwide problem. Among HCV proteins, core antigen (Ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. Herein, we report results of studies on production of the hydrophilic domain of core Ag (2-122) in native conformation by an arabinose induction system in E.coli and the primary characterization of this recombinant protein for applications in diagnosis, immunization and mAb production. Recombinant core (r-Core) was able to detect anti-core antibodies in HCV positive serum samples in a dilution rate of 1/3200. It was also capable to elicit a potent anti-HCV humoral immune response in BALB/c mice. Finally, we established two stable clones of hybridoma which shown to produce specific and sensitive mAbs against the core protein. HCV core was able to elicit a broad range of antibody specificities depending on the immunogen conformation. Therefore, it may be possible to get new mAbs with higher affinities towards native conformation of core Ag.
关键词：HEPATITIS C; HCV CORE PROTEIN; MONOCLONAL ANTIBODY; TOTAL CORE AG; IRAN