文章基本信息

标题：Rapid and retrievable recording of big data of time-lapse 3D shadow images of microbial colonies
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作者：Hiroyuki Ogawa ; Senshi Nasu ; Motomu Takeshige 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep10061
出版社：Springer Nature
摘要：We formerly developed an automatic colony count system based on the time-lapse shadow image analysis (TSIA). Here this system has been upgraded and applied to practical rapid decision. A microbial sample was spread on/in an agar plate with 90 mm in diameter as homogeneously as possible. We could obtain the results with several strains that most of colonies appeared within a limited time span. Consequently the number of colonies reached a steady level (N_stdy) and then unchanged until the end of long culture time to give the confirmed value (N_conf). The equivalence of N_stdy and N_conf as well as the difference of times for N_stdy and N_conf determinations were statistically significant at p stdy meets the requirement of practical routines treating a large number of plates. The difference of N_stdy and N_conf, if any, may be elucidated by means of retrievable big data. Therefore N_conf is valid for official documentation.