文章基本信息

标题：Machine-based method for multiplex in situ molecular characterization of tissues by immunofluorescence detection
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作者：Dmitry Yarilin ; Ke Xu ; Mesruh Turkekul 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep09534
出版社：Springer Nature
摘要：Immunofluorescent staining is an informative tool that is widely used in basic research. Automation of immunostaining improves reproducibility and quality of the results. Up to now, use of automation in immunofluorescent staining was mostly limited to one marker. Here we present tyramide signal amplification based method of multiple marker immunofluorescent detection, including detection of antibodies, raised in the same species, in tissue sections and cultured cells. This method can be beneficial for both basic and clinical research.