文章基本信息

标题：Three CCT domain-containing genes were identified to regulate heading date by candidate gene-based association mapping and transformation in rice
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作者：Li Zhang ; Qiuping Li ; Haijiao Dong 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
DOI：10.1038/srep07663
出版社：Springer Nature
摘要：CCT domain-containing genes generally control flowering in plants. Currently, only six of the 41 CCT family genes have been confirmed to control flowering in rice. To efficiently identify more heading date-related genes from the CCT family, we compared the positions of heading date QTLs and CCT genes and found that 25 CCT family genes were located in the QTL regions. Association mapping showed that a total of 19 CCT family genes were associated with the heading date. Five of the seven associated genes within QTL regions and two of four associated genes outside of the QTL regions were confirmed to regulate heading date by transformation. None of the seven non-associated genes outside of the QTL regions regulates heading date. Obviously, combination of candidate gene-based association mapping with linkage analysis could improve the identification of functional genes. Three novel CCT family genes, including one non-associated ( OsCCT01 ) and two associated genes ( OsCCT11 and OsCCT19 ) regulated the heading date. The overexpression of OsCCT01 delayed flowering through suppressing the expression of Ehd1 , Hd3a and RFT1 under both long day and short day conditions. Potential functions in regulating heading date of some untested CCT family genes were discussed.