文章基本信息

标题：Ultrafast propagation of β-amyloid fibrils in oligomeric cloud
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作者：Hirotsugu Ogi ; Masahiko Fukukshima ; Hiroki Hamada 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep06960
出版社：Springer Nature
摘要：Interaction between monomer peptides and seeds is essential for clarifying the fibrillation mechanism of amyloid β (A β ) peptides. We monitored the deposition reaction of A β _1–40 peptides on immobilized seeds grown from A β _1–42, which caused formation of oligomers in the early stage. The deposition reaction and fibrillation procedure were monitored throughout by novel total-internal-reflection-fluorescence microscopy with a quartz-crystal microbalance (TIRFM-QCM) system. This system allows simultaneous evaluation of the amount of deposited peptides on the surface seeds by QCM and fibril nucleation and elongation by TIRFM. Most fibrils reached other nuclei, forming the fibril network across the nucleus hubs in a short time. We found a fibril-elongation rate two-orders-of-magnitude higher in an oligomeric cloud than reported values, indicating ultrafast transition of oligomers into fibrils.