文章基本信息

标题：Comparative characterization of two intracellular Ca²⁺-release channels from the red flour beetle, Tribolium castaneum
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作者：Yaping Liu ; Chengjun Li ; Jingkun Gao 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep06702
出版社：Springer Nature
摘要：Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP₃Rs) are members of a family of tetrameric intracellular Ca²⁺-release channels (CRCs). While it is well known in mammals that RyRs and IP₃Rs modulate multiple physiological processes, the roles of these two CRCs in the development and physiology of insects remain poorly understood. In this study, we cloned and functionally characterized RyR and IP₃R cDNAs (named TcRyR and TcIP₃R ) from the red flour beetle, Tribolium castaneum . The composite TcRyR gene contains an ORF of 15,285 bp encoding a protein of 5,094 amino acid residues. The TcIP₃R contains an 8,175 bp ORF encoding a protein of 2,724 amino acids. Expression analysis of TcRyR and TcIP₃R revealed significant differences in mRNA expression levels among T. castaneum during different developmental stages. When the transcript levels of TcRyR were suppressed by RNA interference (RNAi), an abnormal folding of the adult hind wings was observed, while the RNAi-mediated knockdown of TcIP₃R resulted in defective larval–pupal and pupal–adult metamorphosis. These results suggested that TcRyR is required for muscle excitation-contraction (E-C) coupling in T. castaneum , and that calcium release via IP₃R might play an important role in regulating ecdysone synthesis and release during molting and metamorphosis in insects.