文章基本信息

标题：Optical coherence tomography: A new strategy to image planarian regeneration
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作者：Yu-Sheng Lin ; Chin-Chou Chu ; Jen-Jen Lin 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep06316
出版社：Springer Nature
摘要：The planarian is widely used as a model for studying tissue regeneration. In this study, we used optical coherence tomography (OCT) for the real-time, high-resolution imaging of planarian tissue regeneration. Five planaria were sliced transversely to produce 5 head and 5 tail fragments. During a 2-week regeneration period, OCT images of the planaria were acquired to analyze the signal attenuation rates, intensity ratios, and image texture features (including contrast, correlation, homogeneity, energy, and entropy) to compare the primitive and regenerated tissues. In the head and tail fragments, the signal attenuation rates of the regenerated fragments decreased from −0.2 dB/μm to −0.05 dB/μm, between Day 1 and Day 6, and then increased to −0.2 dB/μm on Day 14. The intensity ratios decreased to approximately 0.8 on Day 6, and increased to between 0.8 and 0.9 on Day 14. The texture parameters of contrast, correlation, and homogeneity exhibited trends similar to the signal attenuation rates and intensity ratios during the planarian regeneration. The proposed OCT parameters might provide biological information regarding cell apoptosis and the formation of a mass of new cells during planarian regeneration. Therefore, OCT imaging is a potentially effective method for planarian studies.