文章基本信息

标题：Angle-resolved light scattering of individual rod-shaped bacteria based on Fourier transform light scattering
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作者：YoungJu Jo ; JaeHwang Jung ; Jee Woong Lee 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep05090
出版社：Springer Nature
摘要：Two-dimensional angle-resolved light scattering maps of individual rod-shaped bacteria are measured at the single-cell level. Using quantitative phase imaging and Fourier transform light scattering techniques, the light scattering patterns of individual bacteria in four rod-shaped species ( Bacillus subtilis, Lactobacillus casei, Synechococcus elongatus, and Escherichia coli ) are measured with unprecedented sensitivity in a broad angular range from −70° to 70°. The measured light scattering patterns are analyzed along the two principal axes of rod-shaped bacteria in order to systematically investigate the species-specific characteristics of anisotropic light scattering. In addition, the cellular dry mass of individual bacteria is calculated and used to demonstrate that the cell-to-cell variations in light scattering within bacterial species is related to the cellular dry mass and growth.