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标题：Synaptotagmin 1 and Ca²⁺ drive trans SNARE zippering
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作者：Ying Lai ; Xiaochu Lou ; Chuqi Wang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep04575
出版社：Springer Nature
摘要：Synaptotagmin 1 (Syt1) is a major Ca²⁺-sensor that evokes neurotransmitter release. Here we used site-specific fluorescence resonance energy transfer (FRET) assay to investigate the effects of Syt1 on SNAREpin assembly. C2AB, a soluble version of Syt1, had virtually no stimulatory effect on the rate of the FRET at N-terminus of SNARE complex both with and without Ca²⁺, indicating C2AB does not interfere with the initial nucleation of SNARE assembly. However, C2AB-Ca²⁺ accelerated the FRET rate significantly at membrane proximal region, indicating C2AB-Ca²⁺ promotes the transition from a partially assembled SNARE complex to the fusion-competent SNAREpin. Similar enhancement was also observed at the end of the transmembrane domain of SNARE proteins. The stimulatory effect disappeared if there was no membrane or only neutral membrane present.