文章基本信息

标题：A critical re-assessment of DNA repair gene promoter methylation in non-small cell lung carcinoma
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作者：Hongdo Do ; Nicholas C. Wong ; Carmel Murone 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2014
卷号：4
DOI：10.1038/srep04186
出版社：Springer Nature
摘要：DNA repair genes that have been inactivated by promoter methylation offer potential therapeutic targets either by targeting the specific repair deficiency, or by synthetic lethal approaches. This study evaluated promoter methylation status for eight selected DNA repair genes ( ATM , BRCA1 , ERCC1 , MGMT , MLH1 , NEIL1 , RAD23B and XPC ) in 56 non-small cell lung cancer (NSCLC) tumours and 11 lung cell lines using the methylation-sensitive high resolution melting (MS-HRM) methodology. Frequent methylation in NEIL1 (42%) and infrequent methylation in ERCC1 (2%) and RAD23B (2%) are reported for the first time in NSCLC. MGMT methylation was detected in 13% of the NSCLCs. Contrary to previous studies, methylation was not detected in ATM , BRCA1, MLH1 and XPC . Data from The Cancer Genome Atlas (TCGA) was consistent with these findings. The study emphasises the importance of using appropriate methodology for accurate assessment of promoter methylation.