文章基本信息

标题：Modified nucleotides m²G966/m⁵C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon
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作者：Irina V. Prokhorova ; Ilya A. Osterman ; Dmitry E. Burakovsky 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
DOI：10.1038/srep03236
出版社：Springer Nature
摘要：Ribosomes contain a number of modifications in rRNA, the function of which is unclear. Here we show – using proteomic analysis and dual fluorescence reporter in vivo assays – that m²G966 and m⁵C967 in 16S rRNA of Escherichia coli ribosomes are necessary for correct attenuation of tryptophan ( trp ) operon. Expression of trp operon is upregulated in the strain where RsmD and RsmB methyltransferases were deleted, which results in the lack of m²G966 and m⁵C967 modifications. The upregulation requires the trpL attenuator, but is independent of the promotor of trp operon, ribosome binding site of the trpE gene, which follows trp attenuator and even Trp codons in the trpL sequence. Suboptimal translation initiation efficiency in the rsmB/rsmD knockout strain is likely to cause a delay in translation relative to transcription which causes misregulation of attenuation control of trp operon.