文章基本信息

标题：Single domain intrabodies against WASP inhibit TCR-induced immune responses in transgenic mice T cells
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作者：Mitsuru Sato ; Ryoko Sawahata ; Chisato Sakuma 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
DOI：10.1038/srep03003
出版社：Springer Nature
摘要：Intrabody technology provides a novel approach to decipher the molecular mechanisms of protein function in cells. Single domains composed of only the variable regions (V_H or V_L) of antibodies are the smallest recombinant antibody fragments to be constructed thus far. In this study, we developed transgenic (Tg) mice expressing the V_H or V_L single domains derived from a monoclonal antibody raised against the N-terminal domain of Wiskott–Aldrich syndrome protein (WASP), which is an adaptor molecule in immune cells. In T cells from anti-WASP V_H and V_L single domain Tg mice, interleukin-2 production induced by T cell receptor (TCR) stimulation were impaired, and specific interaction between the WASP N-terminal domain and the Fyn SH3 domain was strongly inhibited by masking the binding sites in WASP. These results strongly suggest that the V_H/V_L single domain intrabodies are sufficient to knockdown the domain function of target proteins in the cytosol.