文章基本信息

标题：Functional compensation of glutathione S-transferase M1 (GSTM1) null by another GST superfamily member, GSTM2
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作者：Pritha Bhattacharjee ; Somnath Paul ; Mayukh Banerjee 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
DOI：10.1038/srep02704
出版社：Springer Nature
摘要：The gene for glutathione-S-transferase (GST) M1 ( GSTM1 ), a member of the GST-superfamily, is widely studied in cancer risk with regard to the homozygous deletion of the gene ( GSTM1 null), leading to a lack of corresponding enzymatic activity. Many of these studies have reported inconsistent findings regarding its association with cancer risk. Therefore, we employed in silico , in vitro , and in vivo approaches to investigate whether the absence of a functional GSTM1 enzyme in a null variant can be compensated for by other family members. Through the in silico approach, we identified maximum structural homology between GSTM1 and GSTM2 . Total plasma GST enzymatic activity was similar in recruited individuals, irrespective of their GSTM1 genotype (positive/null). Furthermore, expression profiling using real-time PCR, western blotting, and GSTM2 overexpression following transient knockdown of GSTM1 in HeLa cells confirmed that the absence of GSTM1 activity can be compensated for by the overexpression of GSTM2.