文章基本信息

标题：In situ recognition of cell-surface glycans and targeted imaging of cancer cells
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作者：Xiao-Ding Xu ; Han Cheng ; Wei-Hai Chen 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
DOI：10.1038/srep02679
出版社：Springer Nature
摘要：Fluorescent sensors capable of recognizing cancer-associated glycans, such as sialyl Lewis X (sLe^x) tetrasaccharide, have great potential for cancer diagnosis and therapy. Studies on water-soluble and biocompatible sensors for in situ recognition of cancer-associated glycans in live cells and targeted imaging of cancer cells are very limited at present. Here we report boronic acid-functionalized peptide-based fluorescent sensors (BPFSs) for in situ recognition and differentiation of cancer-associated glycans, as well as targeted imaging of cancer cells. By screening BPFSs with different structures, it was demonstrated that BPFS₁ with a FRGDF peptide could recognize cell-surface glycan of sLe^x with high specificity and thereafter fluorescently label and discriminate cancer cells through the cooperation with the specific recognition between RGD and integrins. The newly developed peptide-based sensor will find great potential as a fluorescent probe for cancer diagnosis.